Fusarium solani pisi cutinase-catalyzed synthesis of polyamides
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چکیده
منابع مشابه
The thermal stability of the Fusarium solani pisi cutinase as a function of pH
We have investigated the thermal stability of the Fusarium solani pisi cutinase as a function of pH, in the range from pH 2-12. Its highest enzymatic activity coincides with the pH-range at which it displays its highest thermal stability. The unfolding of the enzyme as a function of pH was investigated by microcalorimetry. The ratio between the calorimetric enthalpy (DeltaH(cal)) and the van't ...
متن کاملProof for the Production of Cutinase by Fusarium solani f. pisi during Penetration into Its Host, Pisum sativum.
Rabbit antibody to cutinase-I, isolated from Fusarium solani f. pisi, was conjugated to ferritin. With this ferritin-conjugated antibody it was shown that germinating spores of this fungus excreted cutinase during the penetration of the host pisum sativum. This result constitutes the most specific and strongest evidence for an enzymic penetration of a plant cuticle by a pathogen during infection.
متن کاملMechanism by which contact with plant cuticle triggers cutinase gene expression in the spores of Fusarium solani f. sp. pisi.
Spores of the phytopathogenic fungus Fusarium solani f. sp. pisi were shown to produce the extracellular enzyme, cutinase, only when cutin or cutin hydrolysate was added to the spore suspension. Dihydroxy-C(16) acid and trihydroxy-C(18) acid, which are unique cutin monomers, showed the greatest cutinase-inducing activity. Experiments with several compounds structurally related to these fatty ac...
متن کاملIsolation of a Fusarium solani mutant reduced in cutinase activity and virulence.
Fusarium solani isolate T-8 produces an extracellular enzyme, cutinase, which catalyzes the degradation of cutin in the plant cuticle. Cutinase activity can be measured by the hydrolysis of either the artifical substrate, p-nitrophenylbutyrate (PNB), or radioactive cutin containing [14C]palmitic acid. In the present study, the culture filtrate contained basal levels of cutinase when T-8 was gro...
متن کاملCutinase and pectinase in cotton bioscouring: an innovative and fast bioscouring process
This manuscript describes the potential of a cutinase from the fungus Fusarium solani pisi for cotton wax degradation in order to design an efficient low temperature scouring process. The main characteristics, relevant to cotton wax removal with F. solani pisi cutinase are given. The additive effect of cutinase on pectinase was investigated and optimum incubation conditions were determined. Com...
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ژورنال
عنوان ژورنال: European Polymer Journal
سال: 2013
ISSN: 0014-3057
DOI: 10.1016/j.eurpolymj.2012.12.010